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                Position 〉 Information 〉HoloMonitor 〉HoloMonitor M4

                • HoloMonitor M4

                • Model:M4
                • Manufacturer:Phiab
                  • Manufacturer /
                  • Performance /
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                  Phase Holographic Imaging (PHI) specializes in image cytometry. PHI's products enables cell biologists to non-invasively image and quantify living cell populations using time-lapse video microscopy.

                  History

                  Frustrated by the limitations of conventional optical microscopy and inspired by the recent development of high resolution digital image sensors, the founders of PHI set out to develop a holo­graphic microscopy system in early 2001.

                  After building a first functional prototype in collaboration with Lund University and the Com­petence Center for Circuit Design (CCCD), PHI was founded in 2004. Since then a series of successively refined versions have been built and evaluated by local customers.

                  During 2011, the HoloMonitor M3 was internationally launched through a network of regional distributors. In early 2013, the HoloMonitor M3 was followed up by a compact and incubator tolerant version — the HoloMonitor M4.

                  PHI has several internationally granted and pending patents.

                  Microscopy technique Holographic transmission microscopy
                  Sample stage            Fixed
                  Light source External monochromatic laser
                  Camera 1280×1024 pixel CMOS
                  Objective 20×
                  Software HoloStudio M4/Time-laspe or optionally HoloStudio M4/Tracker

                  System performance

                  Lateral resolution 1 µm
                  Field of view ~0.5 mm2
                  Working distance 1.2 mm
                  Digital focusing range ~0.25 mm
                  Exposure time 5 ms, non-scanning
                  Maximum image acquisition rate 1 image/s
                  Sample illumination ~0.1 mW/cm2
                  Maximum height of cell culture chamber 45 mm

                  Computer requirements

                  Windows 7

                  Power requirements

                  Input voltage 100-249 VAC 47-63 Hz
                  Power consumption
                  Microscope unit Maximum 6 W

                  Laser unit

                  Maximum 15 W

                  Physical characteristics

                  Dimensions (L×W×H) 250×160×180 mm
                  Weight 2.5 kg

                  Sample requirements

                  Cells Mono-layer of adherent eukaryotic cells in any of the supported cell culture vessels. Eukaryotic suspension cells require than an IBDI µ-slide is used as cell culture vessel.
                  Cell culture vessels T75, T25, 6-well plate and IBIDI µ-slide

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