Phase Holographic Imaging (PHI) specializes in image cytometry. PHI's products enables cell biologists to non-invasively image and quantify living cell populations using time-lapse video microscopy.
Frustrated by the limitations of conventional optical microscopy and inspired by the recent development of high resolution digital image sensors, the founders of PHI set out to develop a holographic microscopy system in early 2001.
After building a first functional prototype in collaboration with Lund University and the Competence Center for Circuit Design (CCCD), PHI was founded in 2004. Since then a series of successively refined versions have been built and evaluated by local customers.
During 2011, the HoloMonitor M3 was internationally launched through a network of regional distributors. In early 2013, the HoloMonitor M3 was followed up by a compact and incubator tolerant version — the HoloMonitor M4.
PHI has several internationally granted and pending patents.
|Microscopy technique||Holographic transmission microscopy|
|Light source||External monochromatic laser|
|Camera||1280×1024 pixel CMOS|
|Software||HoloStudio M4/Time-laspe or optionally HoloStudio M4/Tracker|
|Lateral resolution||1 µm|
|Field of view||~0.5 mm2|
|Working distance||1.2 mm|
|Digital focusing range||~0.25 mm|
|Exposure time||5 ms, non-scanning|
|Maximum image acquisition rate||1 image/s|
|Sample illumination||~0.1 mW/cm2|
|Maximum height of cell culture chamber||45 mm|
|Input voltage||100-249 VAC 47-63 Hz|
|Microscope unit||Maximum 6 W|
|Maximum 15 W|
|Dimensions (L×W×H)||250×160×180 mm|
|Cells||Mono-layer of adherent eukaryotic cells in any of the supported cell culture vessels. Eukaryotic suspension cells require than an IBDI µ-slide is used as cell culture vessel.|
|Cell culture vessels||T75, T25, 6-well plate and IBIDI µ-slide|